東京大学分子生物学研究所 奥野陽亮先生, 今井祐記先生 (2010)。C57BL/6と129の混合背景。Frt配列で挟まれた配列はActb-FLPeマウス (B6背景) との交配により除去済み。 B6.Cg-Phf2<tm1.2Yima> B6.Cg-Phf2<tm1.2Yima> Phf2 gene floxed mice. Exon 6 was flanked by loxP sites. 条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Diabetes. 2012 Dec 28. Developmental Biology Research Bacteriophage P1 loxP sites, Saccharomyces cerevisiae FRT site, mouse Phf2 genomic DNA PHF2 flox, B6-Phf2 floxed PHF2 flox, B6-Phf2 floxed Homozygote x Homozygote [or Crossing to C57BL/6JJcl] Homozygous mice show no obvious abnormality. Homozygote x Homozygote [or Crossing to C57BL/6JJcl] Homozygous mice show no obvious abnormality. 今井 祐記 Phf2遺伝子のfloxedマウス。Phf2はH3K9me2脱メチル化酵素であり、転写因子のcoactivatorとして働く。本マウスはCre依存的にPhf2を欠損する系統であり、全身性に欠損させた時は、部分的な新生児致死、発育遅延、脂肪組織の委縮を呈する。PHF2の特定組織における機能の研究に有用である。 RBRC05881 In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Diabetes. 2012 Dec 28. C(3〜6か月) Cre/loxP system Yuuki IMAI Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> true M1 [(C57BL/6 x 129)F1] Developed by Dr. Yosuke Okuno and Dr. Yuuki Imai, Institute of Molecular and Cellular Biosciences, The University of Tokyo in 2010. C57BL/6 and 129 mixed. Frt flanked region was removed by crossing with Actb-FLPe mice (B6 background). <a href='https://brc.riken.jp/mus/pcr05881'>Genotyping protocol -PCR-</a> C (3-6 months) FLP/frt system